Technical Data
T8172-60A
TRAF6, CT (TNF Receptor-associated Factor 6, E3 Ubiquitin-protein Ligase TRAF6, Interleukin-1 Signal Transducer, MGC:3310, RING Finger Protein 85, RNF85)
Description:
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Applications:
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
PC-3 or K562 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to 14aa near the C-terminus of human TRAF6.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TRAF6. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takeda K, et al (2003) Annu. Rev. Immunol. 21:335-76. 2. Wu H (2004) Adv. Protein Chem. 68:225-79. 3. Wajant H, et al (2004) Prog. Mol. Subcell. Biol. 34:47-72. 4. Kobayashi T, et al (2003) Immunity. 19:353-63. 5. Cao Z, et al (1996) Nature. 383:443-6. 6. Sato S, et al (2003) J. Immunol. 171:4304-10. 7. Ye H, et al (2002) Nature 2002; 418:443-7.