Technical Data
T8172-60C
TRAF6, CT (TNF Receptor-associated Factor 6, E3 Ubiquitin-protein Ligase TRAF6, Interleukin-1 Signal Transducer, MGC:3310, RING Finger Protein 85, RNF85)
Description:
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-?B. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands (4). It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 0.5ug/ml
Western Blot: 1-2ug/ml
Immunocytochemistry: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
PC-3 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to human TRAF6 (CT).
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TRAF6 (CT). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 2. Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79. 3. Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72. 4. Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63. 5. Cao Z, Xiong J, Takeuchi M, et al. TRAF6 is a signal transducer for interleukin-1. Nature 1996; 383:443-6. 6. Sato S, Sugiyama M, Yamamoto M, et al. Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF) associates with TNF receptor associated factor 6 and TANK binding kinase 1, and activates two distinct transcription factors, NF-kappa B and IFN-regulatory factor-3, in the Toll-like receptor signaling. J. Immunol. 2003; 171:4304-10. 7. Ye H, Aaron JR, Lamothe B, et al. Distinct molecular mechanism for initiating TRAF6 signaling. Nature 2002; 418:443-7.