Technical Data
T8172-65A
TRAF6BP (TRAF6 Binding Protein, TRAF6-binding Protein, TRAF6 Binding Protein T6BP, T6BP, CALCOCO3, PRO0105, Tax1 (Human T cell Leukemia Virus Type I) Binding Protein 1, Tax1 Binding Protein 1, Tax1 Binding Protein, TAX1BP1, TAX1BP1 Protein, TXBP151)
Description:
A TRAF-interacting protein that specifically associates with TRAF6. T6BP shares an overall 50% identity and 59% similarity to a recently identified chicken protein MDP62, which has been implicated in promoting neurite outgrowth. T6BP-TRAF6 complex formation is dependent on the presence of the IL-1 receptor-associated kinase (IRAK).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: Raji

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
This antibody was developed against a portion of amino acids 700-800 of human Traf6BP (Genbank Accession No. AF268075). The sequence used is 94% homologous in mouse.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Researchers are encouraged to titer for their specific applications. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (95% identity with immunogen) and Rat (95% identity with immunogen) due to sequence homology. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Ling L & Goeddel DV T6BP, a TRAF6-interacting protein involved in IL-1 signaling. Proc Natl Acad Sci U S A 97:9567-72 (2000). Uyeda A et al. Chick muscle-derived protein 62: a novel neurite outgrowth promoting protein. Neurosci Lett 284:61-4 (2000). Darnay BG et al. Activation of NF-kappaB by RANK requires tumor necrosis factor receptor-associated factor (TRAF) 6 and NF-kappaB-inducing kinase. Identification of a novel TRAF6 interaction motif. J Biol Chem 274:7724-31 (1999).