Technical Data
TRAIL, Recombinant, Human (TNF-related Apoptosis-inducing Ligand, Apo-2 Ligand, APO2L, Apo-2L, CD253, Protein TRAIL, TL2, Tumor Necrosis Factor Ligand Superfamily Member 10, TNFSF10)
Molecular Biology Storage: -20CShipping: Blue Ice
Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called Apo2 Ligand (Apo2L), is a cytotoxic protein that activates rapid apoptosis in tumor cells, but not at normal cells. The recombinant human TRAIL/Apo2L is a single polypeptide chain containing 168 amino acids and have a molecular mass of 19.6kD.

Recombinant, human TRAIL/Apo2 Ligand expressed in E. coli.

Biological Activity:
The activity is determined by its ability to induce apoptotic cell death in TRAIL-sensitive U343MG cells. The ED50 for the effect is 1-3ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
19.6 kD
Source: E. coli
Purity: ~98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug.
Form: Supplied as a lyophilized powder from 150mM sodium chloride, 50mM sodium phosphate, pH 7.4. Reconstitute with sterile ddH2O to a concentration of 0.5-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.