Technical Data
T8199-02
Transferrin (NT)
Description:
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.

Applications:
Suitable for use in ELISA, Indirect Immuno uorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Fixed, paraf n embedded human brain tissue.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Source:
Ascites

Affinity Constant:
2.85x10e-11M

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.B.799Affinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Puri ed transferrin from pooled human plasma.
Purity:
Purified by Protein G affinity chromatography. 95% (SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the N-terminal domain of human transferrin. Does not crossreact with mouse, rabbit, bovine transferrin or human lactoferrin. Inhibits proliferation of Con-A stimulated peripheral blood lymphocytes (10e-6M)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.