Technical Data
T8199-04M
Transferrin (Tf)
Description:
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of ~79.5kD and a serum concentration range of 18002700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.

Human Transferrin is a glycoprotein with homologous N-terminal and C-terminal iron binding domains. Transferrin(TF) is related to other iron binding proteins including lactoferrin. When human transferrin loaded with iron encounters a transferrin receptor on the surface of a cell, it binds to it and is consequently transported into the cell in a vesicle. The cell will acidify the vesicle, causing human transferrin(TF) to release its iron ions. Each human transferrin molecule has the ability to carry two iron ions in the ferric form (Fe3+).

Human Transferrin levels may be checked in iron deficiency, hemochromatosis and other iron overload disorders. Transferrin is also associated with the innate immune system. Human Transferrin is found in the mucosa and binds iron, thus creating an environment low in free iron, where few bacteria are able to survive.


Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000- 1:10,000
Western Blot: 1:1000- 1:5000 using ECL.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG7C53Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human serum Transferrin.
Purity:
Purified by Prrotein A/G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes human Transferrin at ~80kD. Reacts poorly with rat and mouse Transferrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.