Technical Data
T8199-04V
Transferrin (TF, Trf, Beta-1 Metal-binding Globulin, Serotransferrin, Siderophilin)
Description:
Applications:
Suitable for use in ELISA, Immunoelectrophoresis, Single and Double Radial Immunodiffusion. Other applications not tested.

Recommended Dilution:
Immunoelectrophoresis: 120ul against 2ul serum or equivalent
Double Radial Immunodiffusion (Ouchterlony): Use a rosette arrangement with 10ul in a 3mm diameter center well and 2ul serum samples (neat and diluted) in 2mm diameter peripheral wells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
1ml-20CBlue IceMouseGoat
Concentration:
Not determined
Immunogen:
Purified transferrin isolated from pooled mouse serum.
Purity:
Serum
Form
Supplied as a lyophilized powder. No preservatives added. Reconstitute in 1ml sterile ddH2O. Dilute further in PBS, pH 7.2.
Specificity:
Recognizes mouse transferrin. In immunoelectrophoresis and radial immunodiffusion, a characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.