Technical Data
T8199-08
Transferrin (HRP)
Description:
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.

Applications:
Suitable for use in ELISA, IEP, 2D-IEP, Ouchterlony. Other applications not tested.

Recommended Dilution:
EIA: 1:50,000-1:100,000 (2ug/ml transferrin and TMB as a substrate).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml4C (-20C Glycerol)Blue IceHumanSheep
Concentration:
~5mg/ml
Immunogen:
Human holo-transferrin purified from serum, shown to be homogeneous by SDS-PAGE.
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% Proclin, 300, 0.002% methylisothiazolone, 0.002% bromonitrodioxane. Labeled with HRP.
Specificity:
Recognizes transferrin. Single precipitin arc when tested by IEP and 2D-IEP against human serum. Identity has been confirmed by double diffusion (Ouchterlony) against human serum and an anti-human transferrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.