Technical Data
T8199-11D
Transferrin (Serotransferrin, Beta-1 Metal-binding Globulin, Siderophilin, Tf, Trf) (HRP)
Description:
Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. The major pool of body iron (~85%; 40-50mg/kg) is found in circulating hemoglobin and muscle myoglobin. Iron absorption occurs primarily in the intestine (duodenum) and inversely related to body iron reserve. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs) etc play a key role in iron metabolism.

Transferrin (Tf, human chromosome 3, 679aa), a serum glycoprotein of ~80kD and synthesized in the liver, is the primary protein of inter-organ transport of nonheme iron. Tf can bind two iron atoms. Tf binds to membrane Transferrin receptors (TfRs) and taken up by endocytosis. Iron is released from Tf, within acidic endosomes, into the cytoplasm apparently through the action of DMT1. The apoTf-TfR complex is returned to the cell surface, where, apo-Tf dissociates from TfR at the extracellular pH. The classical TfR, now termed TfR1, is a homodimeric (95kD subunits) type II membrane glycoprotein that binds two molecules of Tf. Human TfR1 (human 760aa; mouse 763aa) has a cytoplasmic domain 1-67aa, 68-88aa TM, and 89-760aa as extracellular domains. A monomeric serum form or soluble TfR1 (80kD) also exists that lacks residues 1-100aa. Recently, a second Tf receptor, TfR2, has been cloned and characterized. TfR2 shares 45% identity with TfR1, and 27% with PMSA. Several variants of Tf have been identified with varying iron binding ability.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000-1:10,000
Western Blot: 1:1,000-1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanGoat
Concentration:
As reported
Immunogen:
Purified human serum Tf protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Specificity:
Recognizes human Transferrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bowman, B. H. et al (1988) Adv. Genet. 25: 1-38. 2. Evans, R. W. et al (1982) Biochem. J. 201: 19-26. 3. MacGillivray, R. T. A et al (1982) PNAS 79: 2504-2508. 4. Park, I. et al (1985) PNAS 82, 3149. 5. Uzan, G. et al (1984) BBRC 119, 273. 6. Yang, F. et al (1984) PNAS 81, 2752-2756. 7. Nelson N et al (1999) EMBO J. 18, 4361(review). 8. Cairo G et al (2000) Biochem. J. 352, 241-250