Technical Data
T8199-13B
Transferrin
Description:
Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions.

Applications:
Suitable for use in ELISA, Immunoblot, (Western and Dot), Immunoprecipitation and conjugation. Other applications not tested.

Recommended Dilution:
ELISA: 1:40,000-1:160,000 (Coat with 1ug transferrin in a standard sandwich ELISA using peroxiddase conjugated rabbit IgG and ABTS as a substrate for 30 min at RT).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~10mg/ml
Immunogen:
Transferrin (Human Serum)
Purity:
Puri ed by delipidation, fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, 40% glycerol.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti rabbit serum, purified and partially purified transferrin (human serum). Crossreactivity against transferrin from other tissues and species may occur by have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.