Technical Data
T8199-13C1
Transferrin (Biotin)
Description:
Transferrin is a single polypeptide chain glycoprotein. It is a member of the iron binding family of proteins. It has a molecular weight of 79.5kD and a serum concentration range of 1800-2700mg/L. It is synthesized in the liver. It consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in hemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy. Serum concentration falls in iron overload, infection and inflammatory conditions.

Biotin/Protein Ratio:
10-20 BAC molecules per rabbit IgG molecule

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunomicroscopy. Other applications not tested.

Recommended Dilution:
ELISA: 1:15,000-1:70,0000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Transferrin [Human Serum]
Purity:
Puri ed from monospeci c antiserum by delipidation, salt fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml polyethylene glycol (PEG-8000), 0.01% sodium azide. Labeled with Biotin.
Specificity:
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-rabbit serum, purified and partially purifed Transferrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.