Technical Data
T8199-13P
Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)
Description:
Transferrin is a glycoprotein with an approximate molecular weight of 76.5kD. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N terminal domains each of which binds 1 ion of ferric iron. The function of this encoded protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.1ug/ml
Western Blot: 1:2000; Predicted molecular weight 77kD.
Optimal dilutions to be determined by the researcher.

Positive Control:
Plasma Transferrin protein

Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanChicken
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-698 from human sertransferrin precursor protein (NP_001054.1) Cellular Localization: Secreted
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human Transferrin. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yang F et al. Proc Natl Acad Sci USA 81:2752-6 (1984).