Technical Data
T8199-13Q
Transferrin (Transferin, TF, Apotransferrin, Beta-1 Metal-binding Globulin, DKFZp781D0156, PRO1400, PRO1557, PRO2086, Serotransferrin Precursor, Siderophilin)
Description:
Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot 1:1000
ELISA 1:2000
Radioimmunoassay 1:100-1:2000
Immunohistochemistry (Paraffin): 10ug/ml
Blocking/Neutralizing 50-200 molar excess
Immunofluorescence 1:10-1:2000
Immunohistochemistry (frozen): 1:10-1:2000
Optimal dilutions to be determined by the researcher.

Cellular Localization: Secreted

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19F314Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineMouse
Concentration:
As reported
Immunogen:
Full length native protein (purified) (Pig).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
This antibody recognizes an epitope located in the N-terminal domain of human transferrin. Species Crossreactivity: Crossreacts with Human, porcine and Rabbit. Does not crossreact with bovine, Carp, canine, Horse or Sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Bartek J et al. Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding. Br J Haematol 59:435-41 (1985). Bartek J et al. Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity. Immunol Lett 4:231-5 (1982).