Technical Data
T8250-05
Transforming Growth Factor alpha, Recombinant, Human (TGFa)
20ug
100ug
Growth Factors, Cytokines Storage: -20CShipping: RT
Transforming Growth Factor-alpha (TGF-alpha) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. TGF-alpha has been shown to play a role in the autocrine growth of certain transformed cells. Human TGF-alpha is a 5.5kD protein containing 50aa residues. The sequence shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds.

Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is 0.2ng/ml, corresponding to a specific activity of 5x10e6units/mg.

Amino Acid Sequence: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
5.5kD
Source: Human, recombinant expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. Reconstitute in sterile ddH2O to 0.1-1mg/ml. Do not vortex.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.