Technical Data
T8250-05
Transforming Growth Factor alpha, Recombinant, Human (TGFa)
20ug
100ug
100ug
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Technical Data |
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T8250-05 |
Transforming Growth Factor alpha, Recombinant, Human (TGFa) |
20ug 100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Transforming Growth Factor-alpha (TGF-alpha) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. TGF-alpha has been shown to play a role in the autocrine growth of certain transformed cells. Human TGF-alpha is a 5.5kD protein containing 50aa residues. The sequence shares approximately 40% sequence homology with EGF, including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. Biological Activity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is 0.2ng/ml, corresponding to a specific activity of 5x10e6units/mg. Amino Acid Sequence: VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA Molecular Weight: 5.5kD |
Source: Human, recombinant expressed in E. coli Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug Concentration: ~1mg/ml Form: Supplied as a lyophilized powder. Reconstitute in sterile ddH2O to 0.1-1mg/ml. Do not vortex. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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