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Technical Data |
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T8250-13 |
Transforming Growth Factor beta1, Latent, Recombinant, Human (TGF beta 1, TGFb1, Camurati Engelmann Disease, CED, Diaphyseal Dysplasia 1 Progressive, DPD1, TGFb1, Differentiation Inhibiting Factor, Cartilage-inducing Factor) |
1ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Biological Activity: The ID50 of TGF-b1 on TF-1 cells in the presence of 2ng/ml GM-CSF was determined as 5-10ng/ml. Quality Control: ATP-Endpoint Assay for Cell Number: TF-1 cells were starved for serum and GM-CSF by overnight incubation in RPMI, 2% FBS. Cells were then incubated in RPMI, 2% FBS, 2ng/ml GM-CSF minus or plus varying concentrations of TGFb1. At the end of three days, cell number was determined using the ATP endpoint assay, ViaLight™. The ID50 was determined as the TGFb1 concentration that gave the half maximal response obtained by cells grown in RPMI, 2% FBS, 2ng/ml GM-CSF alone. (ViaLight™ is a trademark of LumiTech, Ltd.). Storage and Stability: Lyophilized powder may be stored at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 25kD |
Source: Human, recombinant protein expressed in insect cells Purity: Highly Purified ( 98% as estimated by SDS-PAGE, and HPLC). Sterilized through a 0.2um membrane lter and packaged aseptically. Concentration: ~0.02mg/ml Form: Supplies as a lyophilized powder from 5mM sodium citrate, pH 3.5. Reconstitution: May be reconstituted with 50ml sterile water to yield a solution in 5mM sodium citrate, pH 3.5. To dilute further, use PBS, 2mg/ml BSA as a carrier protein. BSA prevents loss of TGF-b1 by adsorption. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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