Technical Data
Transforming Growth Factor beta1, Latent, Recombinant, Human (TGF beta 1, TGFb1, Camurati Engelmann Disease, CED, Diaphyseal Dysplasia 1 Progressive, DPD1, TGFb1, Differentiation Inhibiting Factor, Cartilage-inducing Factor)
Growth Factors, Cytokines Storage: -20°CShipping: RT
Biological Activity: The ID50 of TGF-b1 on TF-1 cells in the presence of 2ng/ml GM-CSF was determined as 5-10ng/ml.

Quality Control:
ATP-Endpoint Assay for Cell Number: TF-1 cells were starved for serum and GM-CSF by overnight incubation in RPMI, 2% FBS. Cells were then incubated in RPMI, 2% FBS, 2ng/ml GM-CSF minus or plus varying concentrations of TGFb1. At the end of three days, cell number was determined using the ATP endpoint assay, ViaLight™. The ID50 was determined as the TGFb1 concentration that gave the half maximal response obtained by cells grown in RPMI, 2% FBS, 2ng/ml GM-CSF alone. (ViaLight™ is a trademark of LumiTech, Ltd.).

Storage and Stability: Lyophilized powder may be stored at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, recombinant protein expressed in insect cells
Purity: Highly Purified ( 98% as estimated by SDS-PAGE, and HPLC). Sterilized through a 0.2um membrane lter and packaged aseptically.
Concentration: ~0.02mg/ml
Form: Supplies as a lyophilized powder from 5mM sodium citrate, pH 3.5.
Reconstitution: May be reconstituted with 50ml sterile water to yield a solution in 5mM sodium citrate, pH 3.5. To dilute further, use PBS, 2mg/ml BSA as a carrier protein. BSA prevents loss of TGF-b1 by adsorption.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.