Technical Data
T8250-13A
Transforming Growth Factor beta 1 (TGFb1)
Description:
TGFb1 (transforming growth factor-beta1) is a family of multifunctional 25kD proteins. TGFb1 was originally identified for its ability to induce the growth of normal rodent fibroblasts in soft agar. It is now known that TGFb1 is a potent growth inhibitor of many normal and transformed cell lines. It regulates normal cell growth, development and tissue remodeling following injury. TGFb1 is produced as latent high molecular weight complexes. The activation of latent TGFb1 is an important step in the regulation of its action.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Immunohistochemistry: 1:200-1:500
Optimal dilutions to be determined by the researcher

Recommended Positive Control:
RAW264.7 cells (WB)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IcePorcineRabbit
Concentration:
~1mg/ml
Immunogen:
E. coli-expressed porcine TGFb1.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes porcine TGFb1. Species Crossreactivity: human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jakowlew, S.B., et al., Nucleic Acids Res 16: 8730 (1988). 2. Miyazono, K. & Heldin, C.H., J. Dermatol. 19: 644-647 (1992). 3. Wyss-Coray, T., et al., Am. J. Pathol. 147(1): 53-67 (1995). 4. Lee, M.S., et al., Am. J. Pathol. 147(1): 42-52 (1995). 5. Grande, J.P.. Proc. Soc. Exp. Biol. Med. 214: 27-40 (1997).