Technical Data
T8250-26
Transforming Growth Factor beta2 Receptor (28NT) (TGFb2R, TGFBR2, Transforming Growth Factor beta Receptor II, AAT3, FAA3, HNPCC6, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR-II, TGFbeta-RII)
Description:
TGF-b is a multifunctional polypeptide growth factor. While specific receptors for this protein have been found on almost all mammalian cell types, the effect of the molecule varies depending on the cell type and growth conditions. Generally, TGF-b is stimulatory for cells of mesenchymal origin and inhibitory for cells of epithelial or neuroectodermal origin. It is a family of regulatory proteins consisting of a number of proteins distantly related. TGF-b1 has been found in the highest concentration in human platelets and mammalian bone, but is produced by many cell types in smaller amounts. TGF-b2 has been found in the highest concentration in porcine platelets and mammalian bone, but again is also produced by many types of cells. The heterodimer, TGF-b1.2, has so far been found only in small amounts in porcine platelets. TGF-b3 has been detected in human, porcine and avian sources, mainly in cells of mesenchymal origin, suggesting a different role for this protein than for TGF-b1 or TGF-b2. TGF-b4 has been detected so far only in chick embryo chondrocytes, and its distribution in other types of cells has not yet been characterized. TGF-b5 has been detected only in Xenopus embryos. TGF-b1, TGF-b2 and TGF-b1.2 appear to be largely equivalent in biological activity, although there appear to be differences in binding to certain types of receptors, and there are a few reports of differential responses to TGF-b1 and TGF-b2.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml detected the p75 TGFbRII receptor in TF-1 cell lysate. WI-38 human diploid fibroblasts can also be used and rat L6 cell lysate using chemiluminescence.
Immunoprecipitation: 1-5ug/sample to immunoprecipitate p75 TGFbRII from lysates of [35S] labeled cells.
Immunocytochemistry: 5-10ug/ml FFPE tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to the first 28 N-terminal residues of the mature human TGF beta receptor (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human p75 TGF beta Type II receptors. Does not crossreact with other identified receptors. Species Crossreactivity: rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lin, et al., Cell 68: 775785 (1992). 2. Hall, F.L., et al., Biochem. J. 316: 303310 (1996). 3. Chen, D., et al., Mol. Cell. Biol. 19: 4684-4694 (1999). 4. Yamada, K., et al., Blood 90: 4832-4841 (1997).