Technical Data
T8250-45
Transforming Growth Factor beta 3, Recombinant, Human (TGFb3)
2ug
10ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

T8250-45 is produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112aa and having a molecular mass of 25.45kD

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.

Biological Activity:
The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is <0.05ng/ml, corresponding to a Specific Activity of 20MU/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TGF-beta 3 as a Reference Standard.

Storage and Stability:
May be stored at -20°C. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
25.45kD
 Source: E. coli
Purity: ~98% as determined by RP-HPLC and SDS-PAGE.
Concentration: ~0.25mg/ml
Form: Supplied as a liquid in 20% ethanol and 0.1% acetate.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Genomic, cDNA, and embryonic expression analysis of zebrafish transforming growth factor beta 3 (tgfbeta3). Dev Dyn 2005 Apr;232(4):1021-30. 2. Rescue of coronal suture fusion using transforming growth factor-beta 3 (Tgf-beta 3) in rabbits with delayed-onset craniosynostosis. Anat Rec A Discov Mol Cell Evol Biol 2003 Oct;274(2):962-71 3. Soft tissue reaction to microgrooved poly-L-lactic acid implants loaded with transforming growth factor beta(3). Tissue Eng 2003 Feb;9(1):117-26 4. [Study on expression of transforming growth factor-beta 3 in the liver tissues] Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 1998 Mar;12(1):61-3 5. Release of bioactive transforming growth factor beta(3) from microtextured polymer surfaces in vitro and in vivo. Tissue Eng 2002 Oct;8(5):853-61 6. Immunohistochemical staining of IGF-I, IGF-binding proteins-1 and -3, and transforming growth factor beta-3 in the umbilical cords of preeclamptic patients. Acta Obstet Gynecol Scand 2002 Aug;81(8):772-80

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.