Technical Data
T8252-90A
Transforming Growth Factor beta Induced, 502-683aa, Recombinant, Human (BIGH3, TGFBI, ig-h3)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
BIGH3, also known as TGFBI and Beta ig-h3, is an extracellular matrix protein induced by transforming growth factor(TGF)-beta 1. BIGH3 protein is involved in cell growth, cell differentiation, wound healing and cell adhesion. In addition, some missense mutations of BIGH3 were identified in families affected with human autosomal dominant corneal dystrophies. BIGH3 gene encodes for a 683 amino-acid protein containing an RGD motif and four internal repeated domains which have highly conserved sequences founded in several species (Fasciclin domain). Recombinant human BIGH3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Endotoxin Level:
1EU/ug (LAL)

Molecular Weight: 19.9kD (182 aa)

Amino Acid Sequence:
MGTVMDVLKG DNRFSMLVAA IQSAGLTETL NREGVYTVFA PTNEAFRALP PRERSRLLGD AKELANILKY HIGDEILVSG GIGALVRLKS LQGDKLEVSL KNNVVSVNKE PVAEPDIMAT NGVVHVITNV LQPPANRPQE RGDELADSAL EIFKQASAFS RASQRSVRLA PVYQKLLERM KH

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
19.9kD
Source: E. coli
Purity: 95% by SDS-PAGE.
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM EDTA, 0.1mM PMSF, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Billings PC., et al. (2002) J Biol Chem. 277, 28003-9. 2. Munier FL., et al. (2002) Invest Ophthalmol Vis Sci. 43, 949-54. 3. Kim JE., et al. (2000) J Biol Chem. 275, 30907-15.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.