Technical Data
TRAP 5 ELISA Kit BioAssay™ (Tartrate-resistant Acid Phosphatase Serum band 5, TRAcP 5, ACP5, PAP, EC
Kits and Assays Storage: 4°C/-20°CShipping: Blue Ice
This Human TRAP 5 Assay is an ImmunoCapture Enzyme-Activity Assay for the quantitative measurement of human TRAP 5 in serum and EDTA plasma. It is intended for research use only.

Calibrator Is a recombinant protein corresponding to human TRAP 5 expressed in Sf9 cells.

Species Crossreactivity: canine, monkey and rabbit.

TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5; EC is a glycoprotein of 35-37kD. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in bone resorption process.
High blood levels of TRAP 5 are usually associated with active bone remodelling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption such as Paget’s disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma, and bilaterally ovariectomized women.
Post-menopausal women have higher levels of serum than post-menopausal women on estrogen replacement therapy. Therefore specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism.

Kit Components:
T8266-96A: Microtiter Strips 1x96 wells, Coated with Anti-TRAP 5 Antibody
T8266-96B: Dissociating Solution, 1x7ml
*T8266-96C: Recombinant TRAP 5 Standard, 0.2U/l, 2x1vial
*T8266-96D: Recombinant TRAP 5 Standard, 2U/l, 2x1vial
*T8266-96E: Recombinant TRAP 5 Standard, 4U/l), 2x1vial
*T8266-96F: Quality Control (3.0-5.5U/l), 2x1vial.
T8266-96G: Dilution Buffer, 1x20ml
T8266-96H: Wash Solution, 10x, 1x100ml
T8266-96J: Substrate Buffer 2x20ml
T8266-96K: Substrate (pNPP) Tablets, 1x4 pcs.
T8266-96L: Stop Solution (0.2M NaOH), 1x13ml

Storage and Stability:
Store *T8266-96C, *T8266-96D, *T8266-96E and *T8266-96F powder at 4°C, liquid for 3 months at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Minkin C. Calcif Tissue Int. 1982, 34, 285-290.2. Lau KH, et al Clin Chem. 1987, 33, 458-462.3. Nakanishi M,et al Clin Chem. 1998, 44, 221-225.4. Halleen JM, et al J Bone Miner Res. 2000, 15, 133-1345.5. Lamp EC, et al . Leuk Lymphoma. 2000, 39, 477-484.6. Nakanishi M, et al Clin Chem. 2000, 46, 469-473.7. Waguespack SG et al J Clin Endocrinol Metab. 2002, 87, 2212-2217.8. Igarashi Y, et al . J Chromatogr B. 2002, 781, 345-358.9. Terpos E, et al Int J Cancer. 2003, 106, 455-457.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.