Technical Data
T8302-50A
TRF4-1 (Topoisomerase Related Function Protein 4 1, Topoisomerase-related Function Protein 4-1, TRF4 1, TRF41, TRF4, DNA Polymerase kappa, DNA Polymerase sigma, LAK1, LAK-1, PAP Associated Domain Containing 7, PAPD7, POLK, Polymerase (DNA-directed) sigma,
Description:
TRF4-1 is a nuclear protein whose expression is cell cycle-regulated at a post-transcriptional level. Two genes, TRF4-1 and TRF4-2, belonging to this family have been identified. TRF4 is also required to maintain a different aspect of higher order chromosome structure, sister chromatid cohesion.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa447-462 of human TRF4-1.
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human TRF4-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Walowsky C, Fitzhugh DJ, Castano IB, Ju JY, Levin NA, Christman MF. J Biol Chem 274 (11):7302-7308 (1999). 2. Castano IB, Brzoska PM, Sadoff BU, Chen H, Christman MF. Genes Dev 10 (20):2564-2576 (1996). 3. Castano IB, Heath-Pagliuso S, Sadoff BU, Fitzhugh DJ, Christman MF. Nucleic Acids Res 24(12): 2404-2410 (1996).