Technical Data
T8320
Triadin
Description:
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR’s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR’s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, Triadin (MW 95kD) transmits electrochemical signals to the SR through direct interaction with both DHPR’s and RyR’s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000. Detects a 95kD protein representing Triadin from rabbit skeletal muscle extracts.
Immunohistochemistry: (Frozen sections) 1:50. Immunohistochemical staining of Triadin in rat skeletal muscle results in striated staining consistent with sarcoplasmic reticulum localization.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG4i341Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbitMouse
Concentration:
As reported
Immunogen:
Purified rabbit fast skeletal muscle Triadin.
Purity:
Ascites.
Form
Supplied as a liquid in PBS with 0.05% sodium azide.
Specificity:
Crossreacts with Rabbit, Rat and Mouse Triadin
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Protasi F et al. J Cell Biol 140 :831-42 (1998). PubMed: 9472035 2.Motoike HK et al J Muscle Res Cell Motil 15 :493-504 (1994). PubMed: 7860698 3. Flucher BE et al. J Cell Biol 123 :1161-74 (1993). PubMed: 8245124 4. Brandt NR et al. J Membr Biol 131 :219-28 (1993). PubMed: 7684084 5.Brandt NR et al. FEBS Lett 299 :57-9 (1992). PubMed: 1544475.