Technical Data
Triadin (TDN, TRDN, dJ166D18.1, DKFZp779I2253, MGC88285, OTTHUMP00000040354, TRISK, TRISK 51, TRISK51, TRISK-51)
The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR?s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR?s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, triadin (MW 95kD) transmits electrochemical signals to the SR through direct interaction with both DHPR?s and RyR?s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRabbitMouse
Not determined
Rabbit skeletal muscle triads.
0.05% Sodium Azide; PBS diluted Ascites
This antibody does not detect triadin from cardiac tissues. Species Crossreactivity: Crossreacts with Mouse and Rabbit. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Campbell KP et al. Identification and characterization of the high affinity [3H]ryanodine receptor of the junctional sarcoplasmic reticulum Ca2+ release channel. J Biol Chem 262:6460-3 (1987). Leung AT et al. Structural characterization of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel from rabbit skeletal muscle. Evidence for two distinct high molecular weight subunits. J Biol Chem 262:7943-6 (1987). Imagawa T et al. Phosphorylation of the 1,4-dihydropyridine receptor of the voltage-dependent Ca2+ channel by an intrinsic protein kinase in isolated triads from rabbit skeletal muscle. J Biol Chem 262:8333-9 (1987).