Technical Data
T8425-04
Triiodothyronine Uptake, Human, BioAssay™ ELISA Kit (T3)
96Tests
Kits and Assays Storage: 4°CShipping: Blue Ice
Triiodothyronine Uptake, Human, BioAssay™ ELISA Kit (T3) permits the measurement of the total amount of binding sites available for the thyroid hormones in human serum or plasma by a microplate enzyme immunoassay.

Triiodothyronine also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Together with thyroxine (T4), T3 exerts a potent influence on the basal metabolic rate. T3 is synthesized from the tyrosine residues of thyroglobin and is found in the serum of normal individuals. Although T3 has a lower serum concentration than T4, it is 5 to 10 times more active.

This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations. In this method, serum reference, patient specimen, or control is first added to a microplate well. Enzyme-T3 conjugate and thyroxine (T4) are added, and then the reactants are mixed. The endogenous binding proteins of the sample react with the thyroxine, but not with the enzyme conjugate. This leads to a higher binding of the enzyme conjugate to the antibody combining sites, reactive for triiodothyronine and thyroxine, immobilized on the well as the binding capacity of the specimen increases. After the completion of the required incubation period, the antibody bound enzyme-triiodothyronine conjugate is separated from the unbound enzyme-triiodothyronine conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color. The employment of several serum references of known unsaturated thyroid hormone binding capacity permits construction of a graph of absorbance and concentration. From comparison to the dose response curve, an unknown specimen's absorbance can be correlated with thyroid hormone binding capacity.

Kit Components:
T8425-04A: Microtiter Plate, 1x96 wells
T8425-04B: Calibrator, 18%U, 1x1ml
T8425-04C:. Calibrator, 24.5%U, 1x1ml
T8425-04D: Calibrator, 34%U, 1x1ml
T8425-04E: Calibrator, 48%U, 1x1ml
T8425-04F. T3 (HRP), 1x1.5ml
T8425-04G: HRP Buffer, 1x13ml
T8425-04H: Substrate A (TMB), 1x7ml
T8425-04J: Substrate B (H2O2), 1x7ml
T8425-04K: Wash Buffer, 50X, 1x20ml
T8425-04L: Stop Solution (1N HCl), 1x8ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months. Opened reagents are stable for 60 days at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Inada, M., et al., J. Clin. Invest. 46: 1442 (1967). 2. Murphy, B., 1968, Radioisotopes in Medicine, U.S. Atomic Energy Commission, Technical Information Center, Tennessee. 3. Hollander, C.S., et al., “Methods of Hormone Radioimmunoassay”, Academic Press, New York (1974). 4. Hamolsky, M.W., et al., J. Clin. Endocrinol. 17: 33 (1957). 5. Hebert, V., U.S. Patent Office #3,442,819 (1971). 6. Mitchell, M.L., et al., J. Clin. Endocrinol. 20: 1474 (1960). 7. Rolleri, E., et al., J. Nucl. Med. 13: 892 (1972). 8. Nusynowitz, M.L., et al., J. Clin. Pathol. 56: 523 (1971). 9. Clark, F., et al., J. Clin. Endocrinol. Metab. 25: 39 (1965). 10. Young, D.S, et al., Clinical Chemistry 21: 3660 (1975).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.