Technical Data
T8466-29D
TRIM29 (Tripartite Motif Containing Protein 29, Tripartite Motif Protein 29, Tripartite Motif Protein TRIM29, Tripartite Motif-containing 29, Ataxia telangiectasia Group D Associated Protein, Ataxia-telangiectasia Group D-associated Protein, ATDC, FLJ3608
Description:
Ataxia- telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing
protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100,000
Western Blot: 1:500-3,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Peptide corresponding to an internal portion of human ATDC protein around lysine 116.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes human. Species Sequence Homology: horse, cattle, chimpanzee and macaque.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kapp LN, Painter RB, Yu LC, van Loon N, Richard CW 3rd, James MR, Cox DR, Murnane JP. Cloning of a candidate gene for ataxia-telangiectasia group D. Am J Hum Genet. 1992 Jul;51(1):45-54. 2.Brzoska PM, Chen H, Zhu Y, Levin NA, Disatnik MH, Mochly-Rosen D, Murnane JP, Christman MF. The product of the ataxia-telangiectasia group D complementing gene, ATDC, interacts with a protein kinase C substrate and inhibitor. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7824-8. 3.Leonhardt EA, Kapp LN, Young BR, Murnane JP. (1994) Nucleotide sequence analysis of a candidate gene for ataxia-telangiectasia group D
(ATDC). Genomics. Jan 1;19(1):130-6.