Technical Data
Triple Sugar Iron Agar (Powder)
Microbiological Grade
Storage RT    Shipping RT
Components (g/L)
Enzymatic Digest of Casein5
Enzymatic Digest of Animal Tissue5
Yeast Enriched Peptone10
Ferric Ammonium Citrate0.2
Sodium Chloride5
Sodium Thiosulfate0.3
Phenol Red0.025
Identification of gram negative enteric organisms based on carbohydrate fermentation and H2S production.

1. Suspend 60 g of the medium in one liter of purified water. 3. Heat with frequent agitation and boil for one minute to completely dissolve the medium.3. Dispense into tubes and autoclave at 121C for 15 minutes.

4. After autoclaving, allow medium to solidify in a slanted position.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.