Technical Data
T8656-21
Anhydrotrypsin, Bovine (Agarose)
1ml
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Technical Data |
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T8656-21 |
Anhydrotrypsin, Bovine (Agarose) |
1ml |
| Molecular Biology | Storage: 4°CShipping: Blue Ice |
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Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C- terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly. Source: Anhydrotrypsin, from bovine pancreas Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Bovine pancreas Purity: ~95% (SDS-PAGE) Concentration: ~1mg/ml Form: Supplied as a liquid in 0.15M sodium chloride, 0.02% sodium azide, pH 4.0. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Kumazaki, T. et al. (1987) J. Biochem. (Tokyo) 102:1539-1546. 2. Blouse, G. E. et al. (2003) Biochemistry 42:12260-12272.
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