Technical Data
Anhydrotrypsin, Bovine (Agarose)
Molecular Biology Storage: 4CShipping: Blue Ice
Immobilized anhydrotrypsin selectively adsorbs peptides with Arg, Lys or AECys (S-aminoethyl cysteine) residues at the C- terminus under weak acidic conditions (1). Strongly acidic conditions are used to elute the bound peptides. Peptides with these amino acids at the N-terminus or internally, as well as free amino acids are not bound. Serine protease inhibitors such as ecotin and PAI-1 may also be purified on this resin (2). May be used repeatedly.

Anhydrotrypsin, from bovine pancreas

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Source: Bovine pancreas
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 0.15M sodium chloride, 0.02% sodium azide, pH 4.0.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kumazaki, T. et al. (1987) J. Biochem. (Tokyo) 102:1539-1546. 2. Blouse, G. E. et al. (2003) Biochemistry 42:12260-12272.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.