		Technical Data
T8663-07	TrkA (Tyrosine Kinase Receptor A, Nerve Growth Factor Receptor, NGFR)

Description:
TrkA is a member of the trk proto-oncogene family and encodes a 140-kilodalton, membrane-spanning protein tyrosine kinase that is the functional receptor for nerve growth factor (NGF). NGF elicits the rapid phosphorylation of gp140trk on tyrosine residues leading to increased c-Fos expression, DNA synthesis and morphologic transformation. A decreased expression of TrkA on the striatal cholinergic neurons has been observed which may contribute, when it reaches a crucial threshold, to the death of cholinergic neurons observed in Alzheimer disease.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry
and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Immunoprecipitation: Detects a band at ~140kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6D813	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

SKNBE neuroblastoma cell line transfected with TrkA (TRK receptor).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in 150mM sodium chloride, pH 7.4, 0.02% sodium azide.

Specificity:

Recognizes an epitope present in the extracellular domain of human TrkA.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tagliabue, E., et al., Int. J. Biol. Markers 14: 68 (1999).