Technical Data
T8665-13C
Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)
Description:
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.

Applications:
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry, Immunoaffinity purification and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.BB.131Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Native troponin complex.
Purity:
Purified by Protein A affinity chromatography. >90% by SDS-PAGE.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Reacts equally with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes an epitope located between amino acid residues 85 and 92 of cTnI. No crossreaction with skeletal muscle troponin I.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.