Technical Data
T8665-16E
Troponin I, Cardiac (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)
Description:
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place Because of which myosin can not bind the actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confirmational changes which leads to dislocation of troponin I and finally tropomyosin leaves the the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character.

Applications:
Suitable for use in Western Blot and Sandwich ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells of Balb/c mice.

Matched Pair:
Detection:
T8665-19B: Troponin I, Cardiac, aa41-49
T8665-16R: Troponin I, Cardiac, aa169-178
Capture:
T8665-16E: Troponin I, Cardiac

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG17E143Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Human troponin complex
Purity:
Purified by Protein A affinity chromatography. >90% (SDS-PAGE).
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes free human cardiac troponin I (cTnl) and cTnl-forming complexes with other troponin components. Not effected by heparin, phosphorylation or oxidation. Recognizes an epitope located between aa83-93 of cTnI. No crossreactivity with skeletal muscle troponin I.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.