Technical Data
T8665-18
Troponin I, Cardiac (cTnI)
Description:
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter “I” is given due to its inhibitory character.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
ELISA: Use as a capture.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken-HRP Rb x Ch
I1906-12 IgY, Chicken-FITC Rb x Ch
I1906-14 IgY, Chicken-AP Rb x Ch

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgYPurified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanChicken
Concentration:
~5mg/ml
Immunogen:
Human Troponin I (Cardiac)
Purity:
Purified
Form
Supplied as a liquid in TBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Troponin I (Cardiac).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.