Technical Data
T8665-19B
Troponin I, Cardiac, aa41-49 (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, Troponin-I, Troponin I Cardiac Muscle, TNNC 1, TNNC1, Troponin I Type 3 Cardiac, TNNI3)
Description:
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter I is given due to its inhibitory character.

Applications:
Suitable for use in ELISA, Western Blot, Immunoaffinity Purification and Immunochromatography. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0 myeloma cells with spleen cells from Balb/c mice.

Source:
Ascites

Recommended Pairs:
Detection:
T8665-20A: Troponin I, Cardiac (cTnI)
Capture:
T8665-16E: Troponin I, Cardiac (cTnI)
T8665-16L: Troponin I, Cardiac (cTnI)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b1.BB.145Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~1.5mg/ml
Immunogen:
Native troponin complex.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes free human cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin, phosphorylation and oxidation. Recognizes an epitope located between aa41-49 of cTnI. Does not crossreact with skeletal muscle troponin I. Species Crossreactivity: monkey, bovine, porcine, goat, canine, rabbit, feline, rat, mouse and fish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.