Technical Data
T8665-22A
Troponin T, Cardiac (cTnT)
Description:
Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.

Applications:
Suitable for use in ELISA, Western Blot and Immunoaffinity Purification. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody. Matched to T8665-22B as the detection or conjugate (label) antibody.

Also suitable for use as the detection or conjugate (label) antibody. Matched to T8665-22C as the capture or solid phase antibody.

Hyrbidoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.BB.154Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Free human Cardiac Troponin T.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Cardiac Troponin T. Recognizes an epitope located betweeen aa95-181. Species Crossreactivity: mouse, rat and canine. Does not crossreact with human skeletal Troponin T.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. FEBS, 315(1): 25–28 (1993).