Technical Data
Tropoelastin (Supravalvular Aortic Stenosis, Williams-Beuren Syndrome, Elastin Precursor, FLJ38671, FLJ43523, SVAS, WBS, WS)
Tropoelastin is a water-soluble molecule with a molecular weight of ~70,000 daltons. Multiple tropoelastin molecules covalently bind together with crosslinks to form the protein elastin that is very prevalent in the body. Tropoelastin is unusual in that it exists in multiple forms, called polymorphs. Tropoelastin is not normally available in its native state, since it becomes crosslinked immediately after its synthesis by the cell and during its export into the extracellular matrix. Tropoelastin confers strength and elasticity to the skin and other organs in the body. A synthetic form of human tropoelastin is made from genetically modified organisms for use in wound healing and topical skin care.

Suitable for Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200-1:500
ELISA: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
500ul-20CBlue IceMouseRabbit
Not determined
Front half of mouse Tropoelastin (containing sequences from exons 6-17).
Supplied as a lyophilized powder. Reconstitute with sterile PBS, 0.01% Thimerosal.
Recognizes mouse Tropoelastin and less strongly with insoluble forms of elastin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mecham, R.P. & Lange, G., Methods Enzymol. 82: 744-759 (1982). 2. Wrenn, D.S., & Meachem, R.P., Methods Enzymol. 144: 246-259 (1987). 3. Liu, X., et al., (2004) Nature Genetics 36: 178-182.