Technical Data
T8668-04
Tropoelastin (Supravalvular aortic stenosis, Williams-Beuren syndrome), Elastin precursor, FLJ38671, FLJ43523, SVAS, WBS, WS)
Description:
Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored. Elastin is particularly abundant in large elastic blood vessels such as the aorta. It is also very important in the lungs, elastic ligaments, the skin, the bladder, and elastic cartilage.
Elastin is composed largely of glycine, proline, and other hydrophobic residues and contains multiple lysine-derived crosslinks, such as desmosines, which link the individual polypeptide chains into a rubberlike network. The hydrophobic regions of the chains, between the crosslinks, are highly mobile. The hydrophobic and crosslinking domains are coded by separate, small (27 to 114 bp) exons that are separated by large introns. The initial translation product is a 72kD polypeptide, designated tropoelastin. Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array.

Applications:
Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000 -1:2000
Western Blot: 1:200 -1:500
Immunoprecipitation: 1:100 -1:200
Immunohistochemistry: 1:50 -1:100
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
500ul-20CBlue IceBovineRabbit
Concentration:
Not determined
Immunogen:
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with sterile PBS.
Specificity:
Recognizes bovine tropoelastin and less strongly with insoluble forms of elastin. Low Species Crossreactivity: human, rabbit, porcine, sheep, canine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.