Technical Data
Tryptic Soy Agar (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Agar, Bacteriological15
Sodium Chloride5
USP medium for total aerobic portion of microbial limit test. A primary media for preparing blood plates.

Tryptic soy agar (TSA) is a general-purpose base medium used for the cultivation of fastidious microorganisms. TSA is a highly nutritious medium consisting of soybean-casein digest. The addition of blood to TSA facilitates the growth of more fastidious microorganisms. Allowing for the interpretation of hemolytic reactions

Beta Hemolysis: Clear zone surrounding the colony indicating complete lysis of the red blood cells.
Alpha Hemolysis: Green discoloration surrounding the colony indicating partial lysis of the red blood cells.
Alpha Prime Hemolysis: Green discoloration followed by a clear zone.
Gamma Hemolysis: No lysis of the red blood cells surrounding the colony.
Note: Hemolysis may vary with the microorganism and/or the blood source.

Directions per Liter: Dissolve 40 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Prepare 5 to 10% blood agar by adding appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 50C.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Tan, homogenous, free flowing powder

Solubility: Yellow, trace to slightly hazy after autoclaving

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.