Technical Data
Media
Tryptone Glucose Extract Agar (Powder)
T8727-20
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/liter
Beef Extract 3.0
Enzymatic Digest of Casein 5.0
Dextrose 1.0
Agar
Total: 24.0g/L
Tryptone Glucose Extract Agar is a standard methods medium for the cultivation and enumeration of microorganisms in dairy and bottled water products. This medium was adopted in 1948 by the American Public Health Association (APHA) for use in testing milk and dairy products. It is also currently the specified medium for the heterotrophic plate count procedure for testing bottled water.

Dehydrated Appearance:
Homogenous, free flowing, light beige

Prepared Appearance:
Trace to slightly hazy, light beige to medium amber

pH: 7.0 0.2


Directions per Liter: Suspend 24g of the medium in one liter of purified water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate container and autoclave for 15 minutes at 121C (15psi). Cool to 50C and dispense into plates. The prepared medium is slightly opalescent and may present a light precipitation.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Test Procedure using Pour Plate Method: 1. Follow the pour plate method as described in Standard Methods (Vanderzant, C., and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C.) 2. Incubate the inoculated medium in a humid atmosphere at 32 1C for 47-49 hours incubation for dairy samples or at 35 0.5C for at least 72 hours for water samples. 3. Count total colonies and record results.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Bowers and Hucker. 1935.Tech. Bull. 228. NY State Agar. Exp. Sta.
2. Prickett. 1928. Tech. Bull. 147. NY State Agar. Exp. Sta.
3. American Public Health Association. 1948. Standard methods for the examination of dairy products, 9th ed. American Public Health Association, Washington, D.C.
4. Vanderzant, C., and D. F. Splittstoesser (eds.). Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C.