Technical Data
Media
Tryptose Phosphate Broth (Powder)
T9058
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/Liter
Tryptose20.0
Sodium Chloride5.0
Sodium Phosphate, Dibasic2.5
Glucose2.0
Total:29.5g/L
Tryptose Phosphate Broth is a buffered dextrose broth for use as an adjuvant to tissue culture media and for the cultivation of fastidious bacteria. Ginsberg, et al., maintained tissue cultures of HeLa cells for at least 10 days in a mixture of 15-25% Tryptose Phosphate Broth, 67.5-77.5% Scheren maintenance solution and 7.5% chicken serum. The cells increased 3-5 fold in number during this period. Smaller quantities of ARD, AD and type 1 poliomyelitis virus could be detected and more ARD virus could be propagated in HeLa cells in the Tryptose Phosphate. Tryptose Phosphate Broth is recommended for the cultivation of streptococci, pneumococci, meningococci and other fastidious organisms. Tryptose Phosphate Broth with added agar and sodium azide is recommended for the isolation of pathogenic streptococci from cheese and other dairy products (2,3). Tryptose Phosphate Broth with added agar is also recommended by the American Public Health Association for the examination of throat swabs and blood for Streptococcus pneumoniae and as a growth medium for pneumococci prior to the bile solubility test.

Certificate of Origin:
The raw animal product used in the manufacturing of Tryptose Phosphate Broth is derived from casein originating in the USA, New Zealand and/or Australia. The animals used received both ante- and post-mortem inspections under certified veterinary care and were inspected to be free of apparent infectious and contagious diseases. During processing of the raw material, a batch is heated to a minimum of 80C for 1 hour including a minimum of 5 minutes at 105C. The product is then dried at a minimum of 140C. BSE (Bovine Spongiform Encephalopathy) is not known to exist in the New Zealand and Australia.


Directions per Liter: Dissolve 29.5 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to beige, homogenous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Ginsberg H.S., et al., Proc. Soc. Exper. Biol. Med. 89: 66-71 (1955). 2. American Public Health Association: Standard Methods for the Examination of Dairy Products. 10th ed., pp. 179, 180, 181 (1953). APHA Inc., New York. 3. Newman, R.W., J. Milk Food Tech. 13: 226-233 (1950). 4. American Public Health Association: Diagnostic Procedures and Reagents. 4th ed., p. 141 (1953). APHA Inc., New York.