		Technical Data
T9101-75	TSG101 (Tumor Susceptibility Gene 101, Tumor Susceptibility Gene 101 Protein, TSG 101, TSG-101, TSG10, Tumor Susceptibility Protein Isoform 3, VPS23)

Description:
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phospho-protein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 0.5-2ug/ml
Western Blot: 1:500-1:2000
Optimal dilutions to be determined by the researcher.

Positive Control:
Molt 4 and HeLa cell lysates.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	9F347	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

A fusion protein containing residues 167-374 of human TSG101 protein.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

Specificity:

Recognizes human TSG101. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Zhu, G., et al. Oncogene. 22(24): 3742-3748, 2003. [Human]2. Garrus, J.E., et al. Tsg101 and the vascular protein sorting pathway are essential for HIV-1 budding. Cell. 107(1): 55-65, 2001. 3. Bishop, N., et al. J. Cell Biol. 157(1):91-101 [Mouse]4. Myers, E.L. and Allen, J.F. Tsg101, . J. Virology. 76(22): 11226-11235, 2002. [Human, Western blot]5. Pornillos, O., et al. HIV gag mimics the Tsg101-recruiting activity of the human Hrs protein. J. Cell Biol. 162(3): 425-434, 2003. [Human, Western blot]6. Bache, K.G., et al. H. J. Cell Biol. 162(3): 435-442, 2003. [Human, Western blot]7. Gottwein, E., et al. THe mason-pfizer monkey virus PPPY and PSAP motifs both contribute to virus release. J. of Virology. 77(17): 9474-9485, 2003. [Western blot, Human]8. Young, T. W., et al. Mol. and Cell. Proteomics. 6: 294-304, 2007.