Technical Data
T9154-01C
Tubulin, alpha, acetyl (Lys40) (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). The Elongator complex catalytic subunit (Elp3) acetylates alpha-tubulin at Lys40 while the histone deacetylase HDAC6 functions as a tubulin deacetylase. This post-transcriptional modification may be required for dynamic cell shape remod-eling, cell motility, tubulin stability and terminal branching of cortical neurons (2,3).

Applications:
Suitable for use in Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IC): 1:800
Immunoprecipitation: 1:50
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10E52Supernatant
SizeStorageShippingSourceHost
40ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic acetylpeptide corresponding to residues surrounding Lys40 of human alpha-Tubulin (P68363).
Purity:
Supernatant
Form
Supplied in liquid 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of human alpha-Tubulin only when acetylated at Lys40, MW: ~52kD. Species Crossreactivity: mouse, rat, monkey, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Westermann, S. & Weber, K., Nat. Rev. Mol. Cell Biol. 4: 938-947 (2003). 2. Creppe, C., et al., Cell 136: 551-564 (2009). 3. Hubbert, C., et al., Nature 417: 455-458 (2002).