Technical Data
Microtubules are filamentous structures that serve in a variety of cellular functions including cell mobility, intracellular transport, and cell division. Microtubules are heterodimers of 2 major proteins, alpha- and beta-tubulins (55kD). The aa sequence of the tubulins is highly conserved evolutionarily. beta-tubulin gene family consists of 15 to 20 dispersed genes, many of which are processed pseudogenes. Within a vertebrate species, the genes are distinguished by their C-terminal region. The aa sequence differences show varying degrees of conservation across species, depending on the isotype. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. The highly acidic C-terminal region may bind cations such as calcium. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth family (zeta-tubulin) which is present only in kinetoplastid protozoa.

Alpha and beta-tubulin Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively. Gamma-tubulin is a ubiquitous phylogenetically Gamma is a low abundance protein present within the cells in both various types of microtubule- organizing centers and cytoplasmic protein complexes. It Interacts with GCP2 and GCP3. Delta-tubulin is associated with the manchette in the elongating spermatid, a specialized microtubule system present during reshaping of the sperm head. It is localized to Centrosome. Centriole. Cytoplasm. Nucleus. it is cytoplasmic and nuclear. Epsilon-tubulin-epsilon is associated with pericentriolar material.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-:1:2,000 (ECL technique)
ELISA: 1:10,000-1:50,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceBovineRabbit
As reported
Purified tubulin, from bovine brain.
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
Recognizes tubulin from bovine brain at ~55kD. Mixture of undefined isotypes. It is highly homologous to tubulin isolated from other eukaryotic sources. Tubulin polymerizes to form structures called microtubules (MTs). Species Crossreactivity: wide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bauman MH (1996) BBRC 219, 238-242. 2. Cowan NJ (1983) Mol Cell Biol. 3, 1738-1745. 3. Olsen JV (2006) Cell 127, 635-648.