Technical Data
T9154-05
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
There are three classes of the ~50kD tubulin protein: alpha, beta and gamma. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP; however, only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.

Applications:
Suitable for use in ELISA, Western Blot, screening of expression libraries, identification of tubulin on fixed, frozen tissue sections and staining of microtubular structures in fixed cells by Immunofluorescence microscopy and by light microscopy using PAP complex. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Immunofluorescence: 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G294Purified
SizeStorageShippingSourceHost
100ug (200ul)4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
Peptide corresponding to the position alpha (65-79) of Tubulin.
Purity:
Purified from ascites by sequential precipitation with caprylic acid and ammonium sulfate solution.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes alpha tubulin of various species. Recognizes a N-terminal structural domain of alpha tubulin. Reacts with peptides that correspond to position alpha (65-79) of Tubulin; band at 50kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Viklicky, V., et al., Cell Biol. Int. Rep. 6: 725 (1982). 2. Draber, P., et al., Eur. J. Cell. Biol. 41: 82 (1986). 3. Draber, P., et al., J. Cell Sci. 92: 519 (1989). 4. Grimm, M., et al., Biochem. Biophys. Acta 914: 83 (1987).