Technical Data
T9154-05D
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
Fusion Partners: Spleen cells from immunized LOU rats were fused with cells of the Y3.Ag.1.2.3 rat myeloma cell line.

Applications:
Immunohistochemistry (frozen)
ELISA
Immunoprecipitation
Western Blot
Radioimmunoassay
Optimal working dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG2a3H3087Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRat
Concentration:
~1mg/ml
Immunogen:
Yeast tubulin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin).The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some crossreactivity with other proteins in certain circumstances, including E. Coli rec A and oxidized actin. Species Crossreactivity: 3H3087 reacts with tubulin from yeast, avian and mammalian.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kilmartin, J.V. et al. (1982). Rat monoclonal anti tubulin antibodies derived by using a new non secreting rat cell line. J. Cell. Biol. 93: 576-582.2. Skinner, R.H. et al. (1991). Use of the Glu-Glu-Phe C-terminal epitop