Technical Data
T9154-05F
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:100 for 20 minutes. No special pretreatment is required for the immunohistochemical staining of formalin/paraffin tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
MAD109 cells, MCF-7 cells; Skin or lung

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide derived from aa426-450 of human tubulin alpha. Epitope: aa426-450. Cellular Localization: Cytoplasmic MW of Antigen: 57kD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human Tubulin alpha. Species Crossreactivity: bovine, porcine, rat, mouse, guinea pig, gerbil, frog and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Banerjee P.P., et al. 2007. Cdc42-interacting protein–4 functionally links actin and microtubule networks at the cytolytic NK cell immunological synapse. J. Exp. Med. 204: 2305 - 2320.