		Technical Data
T9154-07	Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)

Description:
Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.1-1ug/ml of this lot detected a-Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-a-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates a-Tubulin (~50kD).
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	0.N.593	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Purified human brain a-Tubulin. The epitope recognized by this antibody has been mapped to aa426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.

Specificity:

Recognizes a-Tubulin, Mr 50-60kD. Species Crossreactivity: Human, mouse, rat, bovine, porcine, guinea pig and avian.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wang, T. and Lessman, C.A., Comp. Biochem. Physiol. B Biochem. Mol. Biol. 118:421-430, 1997.
2. Breitling, F. and Little, M., J. Mol. Biol. 189:367-370, 1986.3. Roychowdhury, S., et al., Biochem. 32:4955-4961, 1993.4. Blose, S.H., et al., J. Cell Biol. 98:847-858, 1984.5. McKean, P.G., et al. J. Cell Sci. 114:2723-2733, 2001.6. Hadfield, J.A., et al., Prog. Cell Cycle Res. 5:309-325, 2003.