Technical Data
T9154-07
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
Description:
Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 0.1-1ug/ml of this lot detected a-Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-a-Tubulin (0.1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates a-Tubulin (~50kD).
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
Non-stimulated A431 lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.593Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified human brain a-Tubulin. The epitope recognized by this antibody has been mapped to aa426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes a-Tubulin, Mr 50-60kD. Species Crossreactivity: Human, mouse, rat, bovine, porcine, guinea pig and avian.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang, T. and Lessman, C.A., Comp. Biochem. Physiol. B Biochem. Mol. Biol. 118:421-430, 1997.
2. Breitling, F. and Little, M., J. Mol. Biol. 189:367-370, 1986.3. Roychowdhury, S., et al., Biochem. 32:4955-4961, 1993.4. Blose, S.H., et al., J. Cell Biol. 98:847-858, 1984.5. McKean, P.G., et al. J. Cell Sci. 114:2723-2733, 2001.6. Hadfield, J.A., et al., Prog. Cell Cycle Res. 5:309-325, 2003.