Technical Data
Tubulin, alpha (Alpha Tubulin, Tubulin alpha Ubiquitous, H2-Alpha, K-alpha-1, TUBA3, Tubulin alpha 1 Chain, TUBA1, TUBA1A, Tubulin K alpha 1)
The tubulin protein is a major target of drug molecules, and consequently, tubulin inhibitors have attracted great attention as antimitotic antitumor agents for chemotherapeutic use. The effects on tubulin messenger RNA levels and tubulin protein synthesis when treating cells with microtubule-depolymerizing drugs or when directly microinjecting cells with tubulin suggest that non-polymerized tubulin depresses its own synthesis. There are three classes of the ~50kD tubulin proteins: alpha, beta, and gamma. The alpha and beta tubulins form a heterodimer that polymerize into the cylindrical microtubule fibers. Both alpha and beta tubulin bind GTP; however, only beta tubulin hydrolyzes GTP to GDP. This hydrolysis is a process that is linked to tubulin polymerization and microtubule formation. The alpha tubulin isomer can be modified by addition of a C-terminal tyrosine residue. This modification may influence polymerization rates. The gamma tubulin isomer is localized to centrosomes which compose the heart of the microtubule organizing center from which microtubule fibers emanate.

Suitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunofluorescence: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k6A204Affinity Purified
100ug-20CBlue IceHumanMouse
Sarkosyl-resistant filament from sea urchin sperm axonemes.
Purified by Protein A chromatography
Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide
Reacts with all forms of alpha tubulin. The expected molecular weight is ~50.5kD. Reactivity is confirmed with NIH3T3 cells, rat brain, mouse testis, HeLa3 and PtK23 cells. Species Crossreactivity: Human, mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.