Technical Data
T9155-01V
Tubulin beta (Beta-tubulin, Tubulin beta Chain)
Description:
Microtubules are polymers of tubulin, a dimer of two 55kD subunits, designated alpha and beta. Within the microtubule lattice, alpha-beta heterodimers associate in a head-to-tail fashion, giving rise to microtubule polarity. Tubulin binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Distinct human beta-tubulin isotypes are encoded namely M40 and 5 beta, beta 2. The tubulin beta gene maps to human chromosome 6 at 6p21.33. It is expressed in human adrenal gland, colon, kidney, lung and pancreas.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50-1:100
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Positive Control:
Lung

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E382Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceChickenMouse
Concentration:
As reported
Immunogen:
Native chick brain microtubules. Cellular Localization: Cytoplasmic.
Purity:
Purified
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes chicken Tubulin, beta. Species Crossreactivity: bovine, porcine, gerbil, guinea pig, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Berry et al. Biochem Biophys Acta 1012: 45, 1989. 2. Blose et al. J Cell Biol 98: 847, 1984. 3. Roychowdhury et al. Biochemistry 32: 4955, 1993.