Technical Data
T9155-02F
TBB1 (Tubulin beta-1 chain, TUBB1)
Description:
The tubulin family of globular proteins has several members, the most common of which are a-tubulin and -tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells Except in the simplest eukaryotes, tubulin (100kD) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55kD each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and - forms.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Human TBB1 recombinant protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human TBB1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rogowski K., et.al., Cell 137:1076-1087(2009).