		Technical Data
T9155-03R	Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Description:
b-III Tubulin, also known as tubulin b-4, is regarded as a neuron-specific marker. The expression of b-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 8-25ug/ml with immunofluorescence detection. Used in immersion-fixed rat cortical stem cells and immersion-fixed rat cortical neurons.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	TuJ-1	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Rat	Mouse

Concentration:

~0.5mg/ml (after reconstitution)

Immunogen:

Rat brain-derived microtubules

Purity:

Purified by Protein A affinity chromatography

Form

Supplied as a powder lyophilized from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Specificity:

Recognizes rat neuron-specific beta III Tubulin. Species Crossreactivity: human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.