Technical Data
T9155-03R
Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)
Description:
b-III Tubulin, also known as tubulin b-4, is regarded as a neuron-specific marker. The expression of b-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 8-25ug/ml with immunofluorescence detection. Used in immersion-fixed rat cortical stem cells and immersion-fixed rat cortical neurons.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2aTuJ-1Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Rat brain-derived microtubules
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a powder lyophilized from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes rat neuron-specific beta III Tubulin. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.