Technical Data
T9155-12
Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)
Description:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml for 2 hours RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Ls174T and MAD109 cells, Skin or lung.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG33F389Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Purified tubulin, 55kD. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide. See T9155-12X.
Specificity:
Recognizes human Tubulin, beta. Species Crossreactivity: Bovine, rabbit, mouse, chicken, rat, frog, moth and wheat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Lunin, S.M. et al., (2008) Immunol Invest. 37:858-870. 1. Gozes, I. and Barnstable, C.J., Proc. Natl. Acad. Sci. USA 79: 2579 (1982). 2. Matthes, T., et al., J. Immunol. 141: 3135 (1988).