Technical Data
T9155-12
Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, DAQB-47P19.9-001, dJ40E16.7, M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001)
Description:
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kD subunits: alpha and beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml for 2 hours RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Note: No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Ls174T and MAD109 cells, Skin or lung.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG33F389Affinity Purified
SizeStorageShippingSourceHost
500ul4C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified tubulin, 55kD. Cellular Localization: Cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See T9155-12X.
Specificity:
Recognizes human Tubulin, beta. Species Crossreactivity: Bovine, rabbit, mouse, chicken, rat, frog, moth and wheat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Lunin, S.M. et al., (2008) Immunol Invest. 37:858-870. 1. Gozes, I. and Barnstable, C.J., Proc. Natl. Acad. Sci. USA 79: 2579 (1982). 2. Matthes, T., et al., J. Immunol. 141: 3135 (1988).